Composite

Part:BBa_K4031013:Design

Designed by: Yuichiro Ikagawa   Group: iGEM21_Gifu   (2021-10-21)


HHV-6 crRNA 3 for LbCas12a with T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Care must be taken when introducing vectors, particularly the type and placement of and terminators. In order to maximize the function of Cas12a as a crRNA, transcription should be performed without any extra sequences being added to the crRNA.


Source

T7 bacteriophage human herpesvirus 6 ORF 94

References